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WEHI-231

規格:

價格:詢價

編號:B165940

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

WEHI-231

商品貨號

B165940

Organism

Mus musculus, mouse

Cell Type

B lymphocyte, immature

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension, multicell aggregates

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

B cell lymphoma

Strain

(BALB/c x NZB)F1

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

Mineral oil induced tumor in (BALB/c x NZB)F1 mice

Genes Expressed

immunoglobulin

Cellular Products

immunoglobulin

Comments

WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.

Subculturing

Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 10⁵ viable cells/mL. If recovered at 5 X 10⁵ viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 10⁶ viable cells/mL, at which point the viability drops drastically.
After cells recover, expansions can be done at 1 X 10⁵ viable cells/mL by adding media to decrease the cell concentration.
This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 10⁶ cells/mL, the single (non-clustered) and non-viable cells increase along with the amount of debris.
At subculture, break up clusters by gentle pipetting.
Established cultures can be grown in T75 flasks.

Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Isotype

IgM , IgM (surface)

Population Doubling Time

about 20 hours

Name of Depositor

NL Warner, LL Lanier

Deposited As

Mus musculus

References

Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355

Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

mineral oil induced tumor in (BALB/c x NZB)F1 mice

To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml).

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