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WEHI-279
WEHI-279
規格:
價格:詢價
編號:B165943
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
WEHI-279
商品貨號
B165943
Organism
Mus musculus, mouse
Tissue
bone marrow
培養基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, moderate to large multi-cell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
NZC
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Genes Expressed
Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin.
Cellular Products
immunoglobulin
Comments

Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 cells/mL.
Frozen cells may take longer than 2-3 days in complete culture medium to successfully recover from the frozen state.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2 in air atmosphere.
Isotype
IgM (surface); kappa light chain
Name of Depositor
NL Warner, LL Lanier
Deposited As
Mus musculus
References

Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803

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