Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573). 293 cells were transfected with an expression plasmid containing cDNA encoding the rat GABA-A receptor alpha 1, beta 2 and gamma 2 subunits. Genomic integration of the transfected alpha and gamma subunit genes was confirmed; however, the beta subunit sequences were not detected in Northern and Southern blots.
Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits).
Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits).
Wong G, et al. Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119
