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X22
X22
規格:
價格:詢價
編號:B165965
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
X22
商品貨號
B165965
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Comments
Animals were immunized with human brain clathrin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing
    Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 105 and 106 viable cells/mL.
    Medium Renewal: Two to three times weekly
    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype
    IgG1
    Name of Depositor
    FM Brodsky
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Brodsky FM. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J. Cell Biol. 101: 2047-2054, 1985. PubMed: 2415533

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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