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XMMME-001
XMMME-001
規格:
價格:詢價
編號:B165972
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
XMMME-001
商品貨號
B165972
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen
Comments
When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7.
Animals were immunized with M21 human melanoma cells.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10(4) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Isotype
    IgG2a
    Name of Depositor
    Xoma Corporation
    Deposited As
    mouse (B cell); mouse (myeloma)
    U.S. Patent Number
    References

    Scannon PJ, et al. Human melanoma specific immunotoxins. US Patent 4,590,071 dated May 20 1986

    Levy JA, et al. Purification of monoclonal antibodies from mouse ascites eliminates contaminating infectious mouse type C viruses and nucleic acids. Clin. Exp. Immunol. 56: 114-120, 1984. PubMed: 6201307

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