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Z-138
Z-138
規格:
價格:詢價
編號:B167178
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Z-138
商品貨號
B167178
Organism
Homo sapiens, human
Tissue
Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)
培養基
Cell Type
lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
mantle cell lymphoma
Age
70 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hyperdiploid human cell line of male origin with a modal chromosome number of 49 and a 4% polyploidy rate. Most of the cells examined contained the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes present in most of the examined cells included: del(5)(p15), der(9)t(9;?), der(14)t(8;14)?, and add(17p). Overall, the karyology was consistent with published data.
Images
Derivation
The Z-138 cell line was derived from a patient who initially was diagnosed with chronic lymphocytic leukemia (CLL). About 2 years later, the neoplasm had transformed into an aggressive, mature B-cell acute lymphocytic leukemia (B-cell ALL). According to the criteria of the current World Health Organization lymphoma classification, this neoplasm is best classified as mantle cell lymphoma, with blastoid transformation present in the terminal phase of disease.
Clinical Data
70 years
Caucasian
male
The Z-138 cell line was derived from a patient who initially was diagnosed with chronic lymphocytic leukemia (CLL). About 2 years later, the neoplasm had transformed into an aggressive, mature B-cell acute lymphocytic leukemia (B-cell ALL). According to the criteria of the current World Health Organization lymphoma classification, this neoplasm is best classified as mantle cell lymphoma, with blastoid transformation present in the terminal phase of disease.
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Genes Expressed
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7- (verified at ATCC)
Comments
Z-138 cells have a complex karyotype, including the t(11;14)(q13;q32) abnormality, which is common in B-cell neoplasms.
These cells overexpress Cyclin D1.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL. Do not allow the cell concentration to exceed 3 x 106 cells/mL.  

Subcultivation ratio: A subcultivation ratio of 1:5 to 1:15 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: horse serum 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 11
D5S818: 11,13
D7S820: 8
TPOX: 8
THO1: 6
vWA: 15,18
Population Doubling Time
approximately 24 hours
Name of Depositor
Z Estrov
Year of Origin
August 1990
References

Estrov Z, et al . Z-138: a new mature B-cell acute lymphoblastic leukemia cell line from a patient with transformed chronic lymphocytic leukemia. Leuk. Res.:22 (4):341-353, 1998. PubMed 9669839

Medeiros LJ, et al. Z-138 cell line was derived from a patient with blastoid variant mantle cell lymphoma. Leuk. Res. 30(4):497-501, 2006. PubMed 16203034

Tucker CA, et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk. Res. 30: 449-457, 2006. PubMed: 16183118

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