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SU-DHL-2
SU-DHL-2
規格:
價格:詢價
編號:B167179
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SU-DHL-2
商品貨號
B167179
Organism
Homo sapiens, human
Tissue
lymph node
Derived from Metastatic Site: peritoneal effusion
培養基
Cell Type
histiocytic
Product Format
frozen
Morphology
Lymphoblast-like
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
large cell lymphoma; diffuse histiocytic lymphoma
Age
73 years
Gender
Female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
Most of these cells were hyperdiploid, with a sharp modal number of 51 chromosomes; occasional cells in the hypertetraploid range were also seen. A minute marker chromosome was consistently observed, and increased numbers of chromosomes were seen in the A, B, C, and F groups.
Images
CRL-2956 Cell Micrograph
Clinical Data
73 years
Caucasian
Female
Comments
The cells are non-specific esterase and acid phosphatase positive.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
The cells are surface Ig negative (sIG-).
The cells are reported to be E - rosette negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Storage temperature: liquid nitrogen vapor phase

Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 13
D13S317: 11, 12
D7S820: 11, 13
D16S539: 14
vWA: 17
THO1: 6, 9
TPOX: 8
CSF1PO: 12
Amelogenin: X
Name of Depositor
A Epstein
Year of Origin
1974
References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

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