黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > HMEC-1 B167195
HMEC-1
HMEC-1
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B167195
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HMEC-1
商品貨號(hào)
B167195
Organism
Homo sapiens, human
Tissue
dermal endothelium
培養(yǎng)基
Cell Type
dermal microvascular endothelium
Product Format
frozen
Morphology
endothelial-like
Culture Properties
adherent
Biosafety Level
2  [Cells contain SV-40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
newborn
Gender
male
Applications
angiogenesis, leukocyte trafficking, wound healing, inflammation, circulation, tumor growth and metastasis.cancer research, endothelium function, cell trafficking, surface molecule interactions, drug screening, toxicological studies for the pharmaceutical and cosmetic industry
Storage Conditions
liquid nitrogen vapor phase
Derivation
Microvascular endothelial cells isolated from human foreskins were transfected with pSVT vector,  a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen.
Receptor Expression
The cells express von Willebrand's factor (vWF), cell adhesion molecules ICAM-1 and are capable of acetylated LDL uptake. Ref (Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507)
Comments
CRL-3243, HMEC-1, has been well characterized and shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human dermal endothelial cells for many research studies.
Complete Growth Medium
The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium:
  • 10ng/mL Epidermal Growth Factor (EGF)
  • 1 µg/mL Hydrocortisone
  • 10 mM Glutamine
  • fetal bovine serum (FBS) to a final concentration of 10%

Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 
  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor. 
  2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  4. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Subcultivation Ratio: 1:6 to 1:12 is recommended.
Medium Renewal: Do not feed cells with growth medium between subcultures or cells become very tightly attached and will be difficult to disperse with trypsin-EDTA
Cryopreservation
Freeze Medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10, 12
D13S317: 11, 12
D16S539:  11, 12
D5S818: 12
D7S820: 8, 10
THO1: 7
TPOX: 8, 11
vWA: 15, 17
Name of Depositor
Kathryn Kellar, Ph. D.
Year of Origin
November 1990
References

Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507

  • 王經(jīng)理
    手機(jī)號(hào)：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號(hào)：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號(hào)：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號(hào)：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號(hào)：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號(hào)：13626845108
    QQ：972239479
  • 曹經(jīng)理
    手機(jī)號(hào)：18662422060
    QQ：3671869992