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YRp15-leu2delta1 [YRp15/leu2-delta1, p307]

規格:

價格:詢價

編號:B171731

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

YRp15-leu2delta1 [YRp15/leu2-delta1, p307]

商品貨號

B171731

Designations

YRp15-leu2delta1 [YRp15/leu2-delta1, p307]

Depositors

P Hieter

培養基

Biosafety Level

Vector Information

Size (kb): 7.6999998092651370

Vector: YRp15-leu2delta1 (plasmid)

Construction: YRp15

Marker(s):URA3,ampR,SUP11

Construct size (kb): 7.699999809265137

Features: marker(s): ampR, URA3, SUP11
replicon: pMB1, ARS1

Applications

vector for other uses

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/ClaI--6.1, 1.6; SalI--5.9, 1.8; EcoRI/HindIII--6.2, 1.5; HindIII--7.7.

A marker addition/transplacement vector.

Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae.

A 0.6 kb EcoRI/ClaI fragment (blunt-ended) was deleted from a 2.2 kb genomic SalI/XhoI fragment having a SalI linker. The SalI fragment was cloned into YRp15.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Philip Hieter, personal communication

Shipped

freeze-dried

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