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pKD136, pKD8.243A plasmids in <em>E. coli</em>

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編號:B171974

品牌:Mingzhoubio

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DNA

RNA

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產(chǎn)品名稱

pKD136, pKD8.243A plasmids in E. coli

商品貨號

B171974

Designations

pKD136, pKD8.243A plasmids in E. coli

Species

Klebsiella pneumoniae (Schroeter) Trevisan

培養(yǎng)基

Depositors

Purdue Univ. Res. Fndn., A Berry, Purdue Univ. Res. Fndn.

Applications

produces protein 3-dehydroquinate synthase

produces protein 3-dehydroshikimate dehydratase

produces protein 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase

produces protein protocatechuate decarboxylase

produces protein transketolase

Vector

Information on plasmid #1: pKD8.243A
Size (kb): Unknown
Marker: cmlR

Insert component:
Insert size (kb): unknown
Genes: 3-dehydroshikimate dehydratase, aroZ, and protocatechuate decarboxylase, aroY
Source: Klebsiella pneumoniae
DNA: genomic DNA

Vector information:
Vector: unknown
Markers: cmlR

Information on plasmid #2: pKD136
Size (kb): 15.5
Marker: ampR

Insert components (3 genes inserted):
Gene 1: 3-dehydroquinate synthase, aroB
Insert size (kb): 1.65
Source: Escherichia coli
DNA: genomic DNA
Insert ends: SphI

Gene 2: transketolase, tkt
Insert size (kb): 5.0
Source: Escherichia coli RB791
DNA: genomic DNA
Insert ends: BamHI

Gene 3: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, aroF
Insert size (kb): 2.4
Source: Escherichia coli
DNA: genomic DNA
Insert ends: EcoRI linkers

Vector information:
Vector: pBR325
Vector size (kb): 5.7
Marker: ampR

Media

ATCC Medium 1065 plus ampicillin (50 mcg/ml) plus chloramphenicol (50 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Freeze-dried plasmids in E. coli

Comments

Restriction digests of the plasmids in this strain give the following sizes (kb): BamHI--10.0, 5.0, 4.8, 3.4, 1.1, 0.3; EcoRI--9.2, 8.8, 4.0, 2.4; SphI--18.0, 4.4, 1.8.

This strain supports a portion of the biocatalytic conversion of D-glucose into adipate (a reagent in the production of nylon). It converts D-glucose into catechol. Colonies turn brown as catechol accumulates.

The tkt CDS can be recovered as a 2.4 kb EcoRI fragment. The aroF CDS can be recovered as a 5.0 kb BamHI fragment.

The aroB CDS can be recovered as a 1.65 kb SphI fragment.

References

Draths KM, Frost JW. Genomic direction of synthesis during plasmid-based biocatalysis. J. Am. Chem. Soc. 112: 9630-9632, 1990.

Draths KM, Frost JW. Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J. Am. Chem. Soc. 112: 1657-1659, 1990.

Draths KM, Frost JW. Environmentally compatible synthesis of adipic acid from D-glucose. J. Am. Chem. Soc. 116: 399-400, 1994.

Frost JW. Enhanced production of common aromatic pathway compounds. US Patent 5,168,056 dated Dec 1 1992

Pittard J, Wallace BJ. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J. Bacteriol. 91: 1494-1508, 1966. PubMed: 5326114

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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