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pLinkCAT
pLinkCAT
規(guī)格:
價格:詢價
編號:B172555
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pLinkCAT
商品貨號
B172555
Designations
pLinkCAT
Depositors
PL Davies
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 4.5000000000000000
Vector: pLinkCAT (plasmid)
Promoters: Promoter none
Construction: pSV2cat, polylinker
Marker(s):ampR
Construct size (kb): 4.5
Features: insert detection: CAT
marker(s): ampR
promoter: none
replicon: pMB1
terminator: none
enhancer: none
Applications
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.5; BglII--4.5; SstI--4.5; consistent with deposited DNA.
This is a promoter-cloning shuttle plasmid vector with a polylinker which can facilitate both molecular cloning of a DNA fragment as well as controlled deletion of sequence from either end of the insert.
This was constructed from the large AccI/HindIII pSV2CAT fragment and a polylinker with AccI and HindIII ends such that the AccI at the end was lost.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Wu YL, et al. A modified CAT expression vector with convenient cloning sites. Nucleic Acids Res. 18: 1919, 1990. PubMed: 2336378

Shipped
freeze-dried
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