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當(dāng)前位置: 首頁 > ATCC代理 > pA10RP8 [pA10RP8] B172962
pA10RP8 [pA10RP8]
pA10RP8 [pA10RP8]
規(guī)格:
價(jià)格:
編號(hào):B172962
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pA10RP8 [pA10RP8]
商品貨號(hào)
B172962
Designations
pA10RP8 [pA10RP8]
Depositors
P Devilee
培養(yǎng)基
Vector
Construct size (kb): 4.300
Insert
DNA: genomic
Insert lengths(kb): 1.350
Gene product: DNA Segment, repetitive [D10Z1]
Alleles: A2, B2, F2, G2, A2, B2, F2, G2, A1, B1, F1, G1, A1, B1, F1, G1
Insert Size (kb)
1.350
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Plasmid in Escherichia coli
Comments
Restriction digests of the clone give the following sizes (kb): SacI--3.0, 1.35; SacI/PstI--3.0, 0.9, 0.4; PstI--3.8, 0.6; EcoRI--3.36, 0.38 (doublet), 0.3; HindIII--4.3.
There is tight linkage between multiple endocrine neoplasia type 2A (MEN2A) and D10Z1 (theta = 0, LOD score = 12.02).
Data for haplotypes formed by using the A and F morphs improved the LOD score for linkage between multiple endocrine neoplasia type 2A (MEN2A) and D10Z1 (theta = 0, LOD score = 14.06).
There is tight linkage between medullary thyroid carcinoma (MTC) with parathyroid tumors (PTs) and no pheochromocytomas (PHEOs) and D10Z1 (theta = 0, LOD score = 2.86).
The insert contains 4 EcoRI repeat units of 340 bp each. A SacI/PstI double digest should give fragments of 1.0 kb and 0.36 kb from the insert.
High stringency hybridization and washing conditions are necessary due to the repetitive nature of the probe.
The HinfI polymorphism detects the haplotypes at the following frequencies: F+G+ (0.5), F+G- (not seen), F-G+ (0.02), and F-G- (0.48).
References

Devilee P, et al. Chromosome-specific alpha satellite DNA: isolation and mapping of a polymorphic alphoid repeat from human chromosome 10. Genomics 3: 1-7, 1988. PubMed: 3220475

Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709

Carson NL, Simpson NE. Two HinfI RFLPs detected by palphaRP8 at D10Z1. Nucleic Acids Res. 18: 1932, 1990. PubMed: 1970876

Carson NL, et al. The mutation for medullary thyroid carcinoma with parathyroid tumors (MTC with PTs) is closely linked to the centromeric region of chromosome 10. Am. J. Hum. Genet. 47: 946-951, 1990. PubMed: 1978560

Peter Devilee, personal communication

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