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pSK8
pSK8
規格:
價格:詢價
編號:B173045
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSK8
商品貨號
B173045
Designations
pSK8
Species
Homo sapiens, human
培養基
Depositors
M Barbacid, S Katzav
Vector
Construct size (kb): 5.260000228881836
Insert
DNA: cDNA
Insert lengths(kb): 2.299999952316284
Tissue: tumor DNA activated during transfection
Gene product: vav 1 oncogene [VAV]
Insert Size (kb)
2.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.3; ClaI--4.2, 1.37; BamHI--5.25.
The proto-vav gene is not expressed at detectable levels in epithelial, mesenchymal or neuroectodermal cell lines but is readily detected in cell lines of hematopoietic origin.
A partial cDNA clone corresponding to nucleotides 593 to 2765 (with ATG initiator codon at nt 111).
The probe was generated by the depositors while both were at the Frederick Cancer Research Facility, Frederick, MD 21701.
The insert was cloned from a third cycle NIH 3T3 cell transfectant originally transformed with DNA derived from human esophageal carcinoma. The vav oncogene was activated during gene transfer.
References

Katzav S, et al. VAV, a novel human oncogene derived from a locus ubiquitously expressed in hematopoietic cells. EMBO J. 8: 2283-2290, 1989. PubMed: 2477241

Mariano Barbacid, personal communication

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