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pRS405
pRS405
規格:
價格:詢價
編號:B173125
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRS405
商品貨號
B173125
Designations
pRS405
Depositors
P Hieter
培養基
Biosafety Level
1
Host
Shipped in: E. coli DH5alpha
Vector Information
Size (kb): 5.507
Vector: pRS405 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):LEU2,ampR
Construct size (kb): 5.507
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--3.95, 1.55; BglI--3.8, 1.7; PvuII--5.0, 0.45.
A YI-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568

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Shipped
frozen
Shipping Information

?Freeze dried phagemid in E. coli

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