Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2 to 5 days at 35°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
