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pGIKS-BioB
pGIKS-BioB
規格:
價格:詢價
編號:B173253
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGIKS-BioB
商品貨號
B173253
Designations
pGIKS-BioB
GenBank Number

J04423

培養基
Species
Escherichia coli (Migula) Castellani and Chalmers
Depositors
M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct size (kb): 4.1
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector end (5'): XhoI
Vector end (3'): EcoRI
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioB
Gene name: biotin synthetase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: EcoRI linker
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-1041 of the insert correspond to
nucleotides 2012-3052 of J04423.
Insert lengths(kb): 1.10
Gene product: biotin synthetase [bioB]
Insert end (5'): XhoI linker
Insert end (3'): EcoRI linker
Insert Size (kb)
1.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert includes nt 2012 - 3052 of Genbank J04423.
The insert contains the following restriction sites (approximate kb from the 5' end): RsaI--0.33; DdeI--0.58, 0.82; SspI--1.02.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.1; EcoRI/XhoI--3.0, 1.1; XhoI--4.1.
Classification
Enterobacteriaceae, Escherichia
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