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HEL-311 [HEL-31]
HEL-311 [HEL-31]
規格:
價格:詢價
編號:B173318
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HEL-311 [HEL-31]
商品貨號
B173318
Designations
HEL-311 [HEL-31]
GenBank Number

L06850

培養基
Species
Homo sapiens, human
Depositors
KT Taylor, RH Lipsky
Vector
Construct size (kb): 6.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD36
Gene name: CD36 antigen (collagen type I receptor, thrombospondin
receptor)
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 q11.2
Tissue: erythroid leukemia (HEL) cell line
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: L06850
Nucleotides 1-1820 of the insert correspond to
nucleotides 1-1820 of L06850.
Insert lengths(kb): 1.799999952316284
Tissue: erythroid leukemia (HEL) cell line
Gene product: CD36 antigen (collagen type I receptor, thrombospondin receptor) [CD36]
Insert Size (kb)
1.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: frozen
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.05, 1.85, 1.25; EcoRI--2.55, 1.85 (doublet); PstI--2.9, 1.1, 0.67, 0.60, 0.32; PvuII--2.25, 1.85, 1.5, 0.8; XbaI--4.0, 1.85, 0.30.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.68; NdeI--1.10; PstI--0.47, 0.75, 1.29; PvuII--0.56; SphI--1.52; XbaI--1.65.
The insert is an alternatively spliced product containing exon 2b in the 5' untranslated region.
Contains two inserts, tandemly repeated.
Oriented with the 5' end closer to the HindIII site of the polylinker.
References

Taylor KT, et al. Characterization of two alternatively spliced 5'-untranslated exons of the human CD36 gene in different cell types. Gene 133: 205-212, 1993. PubMed: 7693552

Kirk T Taylor, personal communication

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