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M4A4
M4A4
規格:
價格:
編號:B173370
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
M4A4
商品貨號
B173370
Organism
Homo sapiens, human
Cell Type
epithelial
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Cancer
Age
31
Gender
female
Ethnicity
Caucasian
Applications
The parental cell lines CRL-2914 (M4A4) and CRL-2918 (NM2C5) were derived from the human breast cancer cell line, MDA-MB-435. [PubMed: 7683291].
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast.
Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line.
Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
The parental cell lines CRL-2914 (M4A4) and CRL-2918 (NM2C5) were derived from the human breast cancer cell line, MDA-MB-435. [PubMed: 7683291].
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line.
Clinical Data
female
Caucasian
31
Antigen Expression
CD44; Homo sapiens, expressed
Receptor Expression
epidermal growth factor (EGF), expressed
Oncogene
c-myc; Ras; p53 RefUrquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541
Genes Expressed
c-myc; Ras; p53 ,CD44; Homo sapiens, expressed
Comments
The parental cell lines CRL-2914 (M4A4) and CRL-2918 (NM2C5) were derived from the human breast cancer cell line, MDA-MB-435. [PubMed: 7683291]. M4A4 is highly metastatic in immuno-deprived mice, while NM2C5 is weakly or virtually non-metastatic. These well characterized, tumorigenic human isogenic cell lines have dramatically opposite metastatic phenotypes and are ideal for metastatic studies. M4A4 LM3-4 CL 16 GFP (CRL-2917) cell line was derived from a third generation lung metastasis after inoculation of M4A4 GFP cells in a nude mouse mammary gland. The M4A4 GFP (CRL-2915) was developed by the transduction of the GFP gene into M4A4 (CRL-2914) cell line. One of the isolated cell lines, M4A4 LM3-2 GFP (CRL-2916) was derived from a second lung metastasis.
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Note: To avoid phenotypic drift it is recommended to make frozen aliquots of the cells and use each aliquot for only 10 passages. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 x 103 to 3 x 103 viable cells/cm2 is recommended.
  5. Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 8 x 104 and 1 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 2 to 3 times a week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 11,12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18
Population Doubling Time
about 30 hours
Name of Depositor
D Tarin
Year of Origin
1992
References

Bao L, et al. Correlation of VLA-4 integrin expression with metastatic potential in various human tumour cell lines. Differentiation 52: 239-246, 1993. PubMed: 7683291

Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541

Goodison S, et al. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9: 3808-3814, 2003. PubMed: 14506175

Montel V, et al. Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model. Am. J. Pathol. 166: 1565-1579, 2005. PubMed: 15855655

Suzuki M, et al. Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency. Am. J. Pathol. 169: 673-681, 2006. PubMed: 16877365

Montel V, et al. Tumor-stromal interactions reciprocally modulate gene expression patterns during carcinogenesis and metastasis. Int. J. Cancer 119: 251-263, 2006. PubMed: 16482564

Goodison S, et al. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet. Cytogenet. 156: 37-48, 2005. PubMed: 15588854

Hayashi K, et al. Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int. J. Oncol. 22: 623-629, 2003. PubMed: 12579317

Tarin DTumor metastasisIn: Tarin DOxford Textbook of PathologyOxford, United KingdomOxford University Press607-663, 1992

Ross DT, et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174.

Sellappan S, et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res. 64: 3479-3485, 2004. PubMed: 15150101.

Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052.

Ellison G, et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931.

Rae JM, et al., MDA-MB-435 cells are derived from M14 Melanoma cells - a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res. Treat. 104:13-19, 2007. PubMed: 17004106.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541

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