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phpim5R1 [hpim-1, pHPimR1]
phpim5R1 [hpim-1, pHPimR1]
規(guī)格:
價(jià)格:
編號(hào):B173425
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
phpim5R1 [hpim-1, pHPimR1]
商品貨號(hào)
B173425
Designations
phpim5R1 [hpim-1, pHPimR1]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
K Huebner, L Nagarajan
Vector
Construct size (kb): 6.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 3.599999904632568
Tissue: 380 cell line
Gene product: proviral integration, MCF [PIM]
Alleles: A1, A2, B1, B2, C1, A1, A2, B1, B2, C1, C2, C2
Insert Size (kb)
3.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.05; HindIII--3.6, 2.5, 0.9; PstI--2.45, 1.5, 1.2, 1.1, 0.54; BamHI--5.2, 1.2, 0.48; SalI--7.0.
Insert contains BamHI (2) and HindIII (2) sites.
Includes part of intron 4, exon 5, intron 5, exon 6, and 3' flanking sequences.
Cross-hybridizes to mouse Pim-1 gene under stringent conditions.
Vector PyT is a pBR322 derivative with a deletion between EcoRII sites 1058 and 2500.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII.
References

Zoghbi HY, et al. Deletion and linkage mapping of eight markers from the proximal short arm of chromosome 6. Genomics 6: 352-357, 1990. PubMed: 1968423

Nagarajan L, et al. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc. Natl. Acad. Sci. USA 83: 2556-2560, 1986. PubMed: 3458216

Meeker TC, et al. Characterization of the human PIM-1 gene: a putative proto-oncogene coding for a tissue specific member of the protein kinase family. Oncogene Res. 1: 87-101, 1987. PubMed: 3329711

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