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pJA72

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B173427

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pJA72

商品貨號(hào)

B173427

Designations

pJA72

Depositors

SJ Elledge

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 4.8969998359680180

Vector: pJA72 (plasmid)

Promoters: Promoter lac

Construction: pUC, Tn903

Marker(s):TRP1,kanR

Construct size (kb): 4.896999835968018

Features: insert detection: lacZ'
marker(s): kanR, TRP1
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6

Applications

YC-type (centromeric) shuttle vector

shuttle vector

vector for other uses

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.9; XbaI--3.0, 1.7; HindIII--2.7, 2.1; EcoRV--2.8, 1.9.

YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system.

The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - TRP1 - kanR - pMB1 ori.

Media

ATCC^® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin

Growth Conditions

Temperature: 37.0°C

References

Stephen J Elledge, personal communication