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neu c(t)/SP6400
neu c(t)/SP6400
規格:
價格:
編號:B173430
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
neu c(t)/SP6400
商品貨號
B173430
Designations
neu c(t)/SP6400
Species
Rattus norvegicus, rat
培養基
Depositors
RA Weinberg, CI Bargmann
Vector
Construct size (kb): 3.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.4199999868869782
Tissue: neuroblastoma B104 cell line
Gene product: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)( Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2, Neu oncogene, avian erythroblastosis oncogene B 2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogen, tyrosine kinase, receptor HER2) [ERBB2]
Alleles: b, s,
Insert Size (kb)
0.420
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.95, 0.42; HindIII--3.4.
Insert contains StuI (2), BglII, and XhoI sites.
Fragment derives from presumed extracellular portion of the gene. Deduced amino acid sequence is 40% homologous to EGF receptor.
Does not hybridize to EGF receptor gene (c-erbB) even at relatively low stringency (30% formamide). Insert is oriented 3' to 5' relative to the SP6 promoter; transcription yields a probe complementary to neu mRNA.
References

Bargmann CI, et al. The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature 319: 226-230, 1986. PubMed: 3945311

Bargmann CI, et al. Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell 45: 649-657, 1986. PubMed: 2871941

Cornelia I Bargmann, personal communication

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