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Mammalian orthoreovirus 1
Mammalian orthoreovirus 1
規(guī)格:
價(jià)格:
編號(hào):B173679
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Mammalian orthoreovirus 1
商品貨號(hào)
B173679
Classification
Reoviridae, Orthoreovirus
Deposited As
Reovirus type 1
培養(yǎng)基
Agent
Mammalian orthoreovirus 1
Strain
Lang
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Rectal swab from healthy child, Ohio, 1953
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
For passage cell cultures should be harvested after complete involvement of cell sheet. Culture fluid contains a group-specific CF antigen.
REFERENCE STRAIN: Sabin, A.B., Science (Washington, DC) 130: 1387, 1959.
Effect on Host
Yes, in vitro effects: Cytopathic effects (granulated degeneration of cells and then destruction of entire cell sheet)
CPE; granulated degeneration of cells and then destruction of entire cell sheet
Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: LLC-MK2 (ATCC CCL-7.1)

Growth Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Duration: 2-6 days
Name of Depositor
EH Lennette
Source
Rectal swab from healthy child, Ohio, 1953
Year of Origin
1953
References

Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336

Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578

Cross References

Nucleotide (GenBank) : AJ133089 Reovirus type 1 S2 gene, partial, strain Lang.

Nucleotide (GenBank) : M13139 Reovirus (Lang strain) s4 mRNA encoding the major capsid surface protein sigma-3, complete cds.

Nucleotide (GenBank) : M14325 Reovirus (Lang strain) s3 gene encoding the nonstructural RNA-binding protein sigma-NS, complete cds.

Nucleotide (GenBank) : M14779 Reovirus (Lang strain) s1 mRNA encoding the minor capsid cell attachment protein sigma-1a and the nonstructural protein sigma-ibNS, complete cds.

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