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pGL2BIFN
pGL2BIFN
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B173701
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pGL2BIFN
商品貨號(hào)
B173701
Designations
pGL2BIFN
Species
Homo sapiens, human
培養(yǎng)基
Depositors
Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V.
Applications
in another host, produces protein interferon, alpha 2
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGL2B
Intact vector size: 6.600
Type of vector: plasmid
Cloning sites: EcoRV, BglII
Polylinker sites:
Other unique sites: BamHI, HindIII
Construction: pUC12
Host range: Escherichia coli; filamentous fungi
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
promoter: glucoamylase, ->
signal peptide coding region: glucoamylase, ->
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IFNA2
Genomic copy number: unique
Gene name: interferon, alpha 2
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p22
Type of DNA: cDNA
Insert 5' end: DdeI Modification: filled
Insert 3' end: BamHI Modification: filled
Insert size (kb):
Cross references:
Gene product: interferon, alpha 2 [IFNA2]
Target Gene: interferon, alpha 2
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 1.9, 1.2; BamHI--8.0.
The construct has been demonstrated to direct secretion of protein after starch induction in both A. niger and A. nidulans. Should be cotransformed with a plasmid expressing argB for selection.
References

Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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