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BCHE

規格:

價格:詢價

編號:B173847

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

BCHE

商品貨號

B173847

Designations

BCHE

GenBank Number

J02964

培養基

Species

Homo sapiens, human

Vector

Construct size (kb): 5.0

Insert

DNA: cDNA

Insert lengths(kb): 2.299999952316284

Tissue: basal ganglia

Gene product: butyrylcholinesterase(cholinesterase (serum) 1) [BCHE]

Insert Size (kb)

2.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): PstI/SstI--2.8, 2.3; EcoRI--4.7, 0.3; XbaI--5.0; HincII--5.0; BglII--3.3, 1.9.

The insert contains the following restriction sites (kb from the 5' end): BglII--0.1, 1.9; EcoRI--0.3; XbaI--0.9; StuI--1.35; HincII--1.7; SspI--1.8, 2.0, 2.3.

and BglII sites have been introduced 7 nt upstream of the codon for Met -28, and 6 nt downstream of the TAA stop codon. The complete coding sequence can thus be excised as a 1.8 kb BglII fragment.

References

Arpagaus M, et al. Structure of the gene for human butyrylcholinesterase. Evidence for a single copy. Biochemistry 29: 124-131, 1990. PubMed: 2322535

Allderdice PW, et al. The cloned butyrylcholinesterase (BCHE) gene maps to a single chromosome site, 3q26. Genomics 11: 452-454, 1991. PubMed: 1769657

McTiernan C, et al. Brain cDNA clone for human cholinesterase. Proc. Natl. Acad. Sci. USA 84: 6682-6686, 1987. PubMed: 3477799

Oksana Lockridge, personal communication

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