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pPB102
pPB102
規(guī)格:
價格:詢價
編號:B173851
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pPB102
商品貨號
B173851
Designations
pPB102
Depositors
HM Temin
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 8.3299999237060550
Vector: pPB102 (plasmid)
Promoters: Promoter SNV LTR
Construction: pPB101 (ATCC 45012)
Marker(s):ampR
Construct size (kb): 8.329999923706055
Features: marker(s): ampR
promoter: SNV LTR
replicon: pMB1
Applications
expression vector
shuttle vector
Comments
A plasmid shuttle vector constructed from pPB101 (ATCC 45012).
Cloned chicken virus (SNV-60B Sal). Constructed by inserting: BglII deletion of complete SNV (HindIII linkers replace BglII site) in
A plasmid shuttle vector constructed from pPB101 (ATCC 45012) with BglII deletion and HindIII linkers replacing repaired BlgII sites.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Watanabe S, Temin HM. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3: 2241-2249, 1983. PubMed: 6318091

Chen IS, Temin HM. Substitution of 5' helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744

Bandyopadhyay PK, Temin HM. Expression of complete chicken thymidine kinase gene inserted in a retrovirus vector. Mol. Cell. Biol. 4: 749-754, 1984. PubMed: 6325895

O'Rear JJ, et al. Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA. Cell 20: 423-430, 1980. PubMed: 6248244

Shipped
freeze-dried
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