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pGS14-10
pGS14-10
規格:
價格:詢價
編號:B173883
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGS14-10
商品貨號
B173883
Designations
pGS14-10
Species
Homo sapiens, human
培養基
Depositors
DJ Roufa
Applications
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes.
Vector
Construct size (kb): 12.39999961853027
Insert
DNA: genomic
Insert lengths(kb): 10.0
Tissue: HeLa cell line
Gene product: ribosomal protein S14 [RPS14]
Insert Size (kb)
10.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--9.8, 2.8; EcoRI--3.8, 3.2, 3.0, 2.3; PstI--4.8, 3.2, 2.3, 1.8; SalI--13.0; BamHI--4.6, 4.1, 3.8.
The insert extends from an EcoRI site to the HindIII site (about 1.5 to 11.5 kb). It includes 3 copies of a 10 bp sequence (-69, -29, +1) found in other ribosomal protein genes.
The coding sequence starts in exon 2. Introns 3 and 4 contain Alu-homologous repeats. The 3' flanking sequence contains 10 bp complementary to U1 RNA.
A 260 bp AluI/PstI fragment in intron 3 has been shown to be single copy and useful for mapping purposes. It shows only slight cross-reaction with CHO cell (Chinese hamster) DNA.
The fragment cloned was an EcoRI/HindIII fragment to which a HindIII linker was added. Thus the clone contains both EcoRI and HindIII sites at its 5' end and a HindIII site at the 3' end. There are several internal EcoRI sites.
References

Diaz JJ, Roufa DJ. Fine-structure map of the human ribosomal protein gene RPS14. Mol. Cell. Biol. 12: 1680-1686, 1992. PubMed: 1549121

Rhoads DD, et al. Primary structure of human ribosomal protein S14 and the gene that encodes it. Mol. Cell. Biol. 6: 2774-2783, 1986. PubMed: 3785212

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