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pJM6 phagemid in E. coli

規格:

價格:詢價

編號:B173948

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pJM6 phagemid in E. coli

商品貨號

B173948

Designations

pJM6 phagemid in E. coli

Depositors

TW Jeffries

培養基

Biosafety Level

Vector Information

Size (kb): 5.744

Vector: pJM6 (phagemid)

Promoters: Promoter for in vitro transcription T7

Construction: pJM4, pBluescriptIIKS+

Marker(s):URA3,ampR

Construct size (kb): 5.74399995803833

Features: marker(s): URA3
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS2
replicon: f1
replicon: pMB1
MCS: SacI/.../EcoRI

Applications

shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--2.9 doublet; PstI/XhoI--2.9 doublet; XbaI/XhoI--2.9 doublet; HindIII--6.0; XhoI--6.0.

The insert contains the following restriction sites (approximate kb from the 5' end): HpaII--0.76, 1.08; BsmI--0.97; HindIII--1.77; AgeI--1.94.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063

Shipped

freeze-dried

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