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pT7/Nco
pT7/Nco
規(guī)格:
價格:詢價
編號:B174053
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pT7/Nco
商品貨號
B174053
Designations
pT7/Nco
Depositors
RS Haun
培養(yǎng)基
Other IDs

Nucleotide (GenBank) : U25269 Expression cloning vector pT7/Nde, complete sequence.

Biosafety Level
1
Vector Information
Size (kb): 2.9000000953674320
Vector: pT7/Nco (plasmid)
Promoters: Promoter SP6
Construction: pGEM-3, pET-3d
Marker(s):ampR
Construct size (kb): 2.900000095367432
Features: marker(s): ampR
promoter: SP6
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...XbaI
ribosome-binding site: gene 10
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--2.9; BamHI--2.9; NcoI--2.9.
The NcoI site (CCATGG) includes the initiator methionine codon. The NcoI cloning site can also be used in conjunction with the downstream BamHI site.
Differs from pT7/NdeI (ATCC 87050) only in the cloning site and reading frame for the insert.
Expression vector, maintained at high copy number.
The order of the major features of the plasmid is: SP6 - gene 10 RBS - T7 - NcoI - BamHI - pMB1 ori - ampR.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566

Shipped
freeze-dried
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