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pSVLfur
pSVLfur
規格:
價格:
編號:B174279
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSVLfur
商品貨號
B174279
Designations
pSVLfur
GenBank Number

X17094

培養基
Species
Homo sapiens, human
Depositors
WJ Van De Ven
Applications
in another host, produces protein paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme
Vector
Construct size (kb): 9.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSVL
Intact vector size: 4.889
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI
Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40 late
replicon: pMB1, SV40
terminator: SV40 late polyadenylation
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PACE
Other gene symbols: FUR
Genomic copy number: unique
Gene name: paired basic amino acid cleaving enzyme (furin, membrane
associated receptor protein)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15 q26.1
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.1
Cross references: DNA Seq. Acc.: X17094
Insert lengths(kb): 4.099999904632568
Gene product: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)( paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme) [FUR]
Target Gene: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)
Insert Size (kb)
4.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 2.4; EcoRI--4.1, 2.7, 2.2; KpnI--3.9, 3.4, 1.8; PstI--3.1, 2.5, 2.0, 1.3; SmaI--5.8, 3.4.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.48, 2.42, 3.62; KpnI--0.58, 3.97; BamHI--1.84.
Contains the complete coding sequence (nt 217-2601).
Constructed by introducing an EcoRI site in the SmaI site of the vector.
References

van den Ouweland AM, et al. Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2. Nucleic Acids Res. 18: 664, 1990. PubMed: 2408021

Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280

van de Ven WJ, et al. Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes. Mol. Biol. Rep. 14: 265-275, 1990. PubMed: 2094803

Wim J Van De Ven, personal communication

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