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當前位置: 首頁 > ATCC代理 > pT223 [D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223] B174300
pT223 [D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223]
pT223 [D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223]
規格:
價格:
編號:B174300
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pT223 [D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223]
商品貨號
B174300
Designations
pT223 [D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223]
GenBank Number

M11722

培養基
Species
Homo sapiens, human
Depositors
Henry M. Jackson Foundation, FJ Bollum, Henry M. Jackson Foundation
Applications
produces protein terminal deoxynucleotidyl transferase
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.736999988555908
Tissue: lymphoblast KM-3 cell line
Gene product: terminal deoxynucleotidyl transferase [TDT]
Target Gene: terminal deoxynucleotidyl transferase
Insert Size (kb)
1.737
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; PvuII--2.3, 1.1, 0.6, 0.4; BamHI/EcoRI--2.6, 1.1, 0.7; HindIII--4.4; BglII--4.4.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.64; XmnI--0.17, 0.63, 0.79; PvuII--0.9, 1.25.
The insert corresponds to nt 332-2068 of the published sequence, including all of the CDS (nt 329-2068) except for the initiation ATG.
The recombinant fusion protein lacks the human N-terminal methionine and includes 15 amino acids encoded by the vector. It is immunoreactive and demonstrates catalytic activity.
References

Peterson RC, et al. Molecular cloning of human terminal deoxynucleotidyltransferase. Proc. Natl. Acad. Sci. USA 81: 4363-4367, 1984. PubMed: 6087320

Peterson RC, et al. Expression of human terminal deoxynucleotidyl transferase in Escherichia coli. J. Biol. Chem. 260: 10495-10502, 1985. PubMed: 2863268

Bollum FJ, et al. Recombinant DNA molecules for producing terminal transferase-like polypeptides. US Patent 5,037,756 dated Aug 6 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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