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pAAV/Ad [pAd/AAV]
pAAV/Ad [pAd/AAV]
規格:
價格:詢價
編號:B174354
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAAV/Ad [pAd/AAV]
商品貨號
B174354
Designations
pAAV/Ad [pAd/AAV]
GenBank Number

J01901

L43079

培養基
Species
Adenovirus
Depositors
Trustees of Princeton University, TE Shenk, Trustees of Princeton University
Applications
in another host, produces protein capsid protein
in another host, produces protein replication protein
integration helper plasmid
Vector
Construct size (kb): 7.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 4.300000190734863
Gene product: capsid protein [cap]
Target Gene: , capsid protein, replication protein
Insert Size (kb)
4.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): ClaI & EcoRI--3.4, 2.6, 1.8; HindIII--6, 1.8; XbaI--4.2, 3.6.
AAV2 insert contains the following restriction sites (approximate kb from the 5' XbaI site): BamHI--0.85; BglI--0.35, 4.2; EcoRI--1.6, 1.8; HindIII--1.7; KpnI--1.7, 4.0; PstI--0.3, 1.75, 4.05; SacI--0.6; SalI--1.25; XhoI--2.05, 2.2.
Helper virus for packaging helper-free recombinant AAV. Plasmid provides all trans functions (replication and capsid proteins) necessary for packaging AAV.
Useful for creating recombinant AAV in conjunction with the cloning vector pAAV/neo (ATCC 68065).
Constructed by replacing the inverted terminal repeats of the AAV2 genome with inverted repeats from adenovirus type 5. The poison sequence of pBR322 was also removed.
The AAV portion of the insert can be excised by digestion with XbaI.
References

Shenk TE, et al. Helper-free stocks of recombinant adeno-associated virus vectors. US Patent 5,436,146 dated Jul 25 1995

Samulski RJ, et al. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 63: 3822-3828, 1989. PubMed: 2547998

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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