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fd-tet
fd-tet
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B174501
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
fd-tet
商品貨號(hào)
B174501
Designations
fd-tet
Depositors
GP Smith
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 9.2
Vector: fd-tet (phage, lambda - replacement)
Construction: phage fd, Tn10
Marker(s):tetR
Construct size (kb): 9.2
Features: marker(s): tetR
replicon: fd
Applications
vector permitting production of single-stranded DNA
Comments
Distributed as infected E. coli JM101 (ATCC 33876).
Has been used as the starting material to construct a vector expressing immunoglobulin variable region-gene III fusion proteins which can be screened by antigen with the intact bacteriophage ("phage antibodies").
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Zacher AN, et al. A new filamentous phage cloning vector: fd-tet. Gene 9: 127-140, 1980. PubMed: 6247241

Nelson FK, et al. Filamentous phage DNA cloning vectors: a noninfective mutant with a nonpolar deletion in gene III. Virology 108: 338-350, 1981. PubMed: 6258292

McCafferty J, et al. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552-554, 1990. PubMed: 2247164

Shipped
frozen
Shipping Information

Shipped: ?Frozen glycerol stock of fd-tet infected E. coli

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