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Y-227
Y-227
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B174517
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Y-227
商品貨號(hào)
B174517
Designations
Y-227
Species
Homo sapiens, human
培養(yǎng)基
Depositors
U Muller
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.799999952316284
Tissue: 49,XXXXY cell line
Gene product: DNA Segment, single copy [DYS185]
Insert Size (kb)
2.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.2; AvaI--7.2; PstI--7.2; EcoRI--7.2; HindIII--4.4, 2.8.
Y-227 hybridizes to a BssHII fragment of 2.7 Mb also detected by Y-280. Y-227 hybridizes to a NotI fragment of 3 Mb which is not detected by Y-280.
In HindIII digests, Y-227 hybridizes to a 2.8 kb Y-specific fragment and a smaller non-Y fragment. Each is single copy. The Y-specific band was detected in 8 of 11 46,XX males, but not in 2 46,XX hermaphrodites or 1 46,XY gonadal dysgenesis patient.
Isolated from a flow-sorted human Y chromosome library enriched for DNA from the Y euchromatic portion.
References

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

Muller U, Lalande M. A physical map of the human Y-chromosome short arm. Genomics 7: 517-523, 1990. PubMed: 1974877

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