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p67-10
p67-10
規格:
價格:詢價
編號:B174672
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
p67-10
商品貨號
B174672
Designations
p67-10
GenBank Number

M32011

培養基
Species
Homo sapiens, human
Applications
produces protein neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) neutrophil cytosolic factor 2
Vector
Construct size (kb): 5.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NCF2
Genomic copy number: unique
Gene name: neutrophil cytosolic factor 2 (65kD, chronic granulomatous
disease, autosomal 2)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 cen-q32
Tissue: HL-60 cell line (promyelocytic), differentiated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.16
Cross references: DNA Seq. Acc.: M32011
Insert lengths(kb): 2.160000085830689
Tissue: HL-60 cell line (promyelocytic), differentiated
Gene product: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)(neutrophil cytosolic factor 2) [NCF2]
Alleles: A1, A2
Target Gene: neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
Insert Size (kb)
2.160
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; SstI--3.7, 0.9, 0.41; BamHI--4.0, 1.0; BglII--5.1; PstI--5.1.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.84, 1.22; BamHI--0.97; BglII--0.78; SmaI--1.17.
Contains the complete coding sequence (nt 68-1648).
Detects a transcript of 2.4 kb.
The polymorphism was detected with a 0.84 kb EcoRI/SacI fragment.
Enzymes(s) not detecting polymorphism: AvaI, BamHI, EcoRI, KpnI, PstI, SacI, XhoI.
References

Francke U, et al. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. PubMed: 2393022

Leto TL, et al. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60(c-src). Science 248: 727-730, 1990. PubMed: 1692159

Kenney RT, Leto TL. A HindIII polymorphism in the human NCF2 gene. Nucleic Acids Res. 18: 7193, 1990. PubMed: 1979859

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