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phins311
phins311
規格:
價格:
編號:B174703
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
phins311
商品貨號
B174703
Designations
phins311
Species
Homo sapiens, human
培養基
Depositors
GI Bell
Applications
The presence of the extra copy of pBR322 does not appear to generate any hybridization problems because the entire plasmid has been used as a probe.
Vector
Construct size (kb): 12.89999961853027
Insert
DNA: genomic
Insert lengths(kb): 8.600000381469727
Gene product: insulin-like growth factor 2 [IGF2]
Alleles: A1, A2
Insert Size (kb)
8.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 4.6; BamHI--6.6, 2.2, 2.0, 1.8, 0.9, 0.62; HindIII--7.4, 5.3, 0.65, 0.48, 0.36; PstI--7.0, 3.65, 0.96, 0.87, 0.68, 0.37, 0.22; SalI--17.0.
Codominant segregation of the BamHI RFLP demonstrated in 3 families.
Detects genomic restriction fragments of the following sizes (kb): owl monkey HindIII--7.4, 4.6, 2.7; mouse HindIII--10.5, 4.0.
Subcloned from lambda hINS-3. Contains 5' flanking sequences, exon 1 encoding part of the 5' untranslated region of IGF2, and part of the adjacent intron 1.
Allele frequencies are different (A1 = 0.79, A2 = 0.21) in a Chinese population.
The presence of the extra copy of pBR322 does not appear to generate any hybridization problems because the entire plasmid has been used as a probe.
The construct appears to include 2 copies of the vector with the 8.6 kb insert from lambdahINS-3 because the intensity of the 4.4 kb band in the EcoRI digest is the same as the 8.8 kb band and there is an extra band in the SalI digest.
Enzyme(s) not detecting polymorphism: ApaI, BanI, BglI, BglII, DraI, KpnI, MboII, MspI, PvuII, RsaI, SstI, XbaI.
References

Ma NS, et al. Homologs of eleven loci on human chromosome 11 assigned to two owl monkey chromosomes. Cytogenet. Cell Genet. 56: 206-211, 1991. PubMed: 2055119

Xiang K, et al. BamHI RFLP at the insulin-like growth factor II (IGF2) locus on chromosome 11. Nucleic Acids Res. 15: 7655, 1987. PubMed: 2889194

Bell GI, et al. Isolation of the human insulin-like growth factor genes: Insulin-like growth factor II and insulin genes are contiguous. Proc. Natl. Acad. Sci. USA 82: 6450-6454, 1985. PubMed: 3901002

Graeme I Bell, personal communication

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