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Pst/Sma2

規(guī)格:

價格:詢價

編號:B174762

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Pst/Sma2

商品貨號

B174762

Designations

Pst/Sma2

GenBank Number

D00657

培養(yǎng)基

Species

Red clover mottle virus

Depositors

M Shanks

Vector

Construct size (kb): 10.10000038146973

Insert

DNA: cDNA

Insert lengths(kb): 2.900000095367432

Gene product: 24K protease

Insert Size (kb)

2.900

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.4, 1.7, 1.0; PvuII--6.8, 1.6, 0.9, 0.7; PstI--10.5; PstI/EcoRI--8.5, 1.6; PstI/SstI--7.2, 1.7, 1.2; TaqI--2.0, 1.4, 1.05, 0.95, 0.8, 0.7, 0.6 + smaller.

The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--1.3, 2.1; TaqI--1.4, 2.8; BamHI--1.7, 2.2.

The 5' end of the insert corresponds to the PstI site approximately 1.6 kb from the 5' end of the RNA.

Classification

Comovirus

References

Shanks M, Lomonossoff GP. The primary structure of the 24K protease from red clover mottle virus: implications for the mode of action of comovirus proteases. J. Gen. Virol. 71: 735-738, 1990. PubMed: 2179468

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