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pT7L-21

規格:

價格:詢價

編號:B174887

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pT7L-21

商品貨號

B174887

Designations

pT7L-21

Species

Tetrahymena thermophila Nanney and McCoy

培養基

Depositors

Univ. Colorado, TR Cech, Univ. Colorado

Applications

After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme.

Vector

Construct size (kb): 3.200000047683716

Insert

DNA: genomic

Insert lengths(kb): 0.5

Gene product: ribozyme having endoribonuclease activity toward recognition sequence CUCU (RNA, ribosomal)

Insert Size (kb)

0.5

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.5; EcoRI--3.2; HindIII--3.2.

Distributed in aliquots of 200 ng dried.

Constructed by inserting the SphI/HindIII fragment [intervening sequence (IVS) minus the first 44 bp] from pBGST7 (ATCC 40288) into pUC18, and adding a synthetic EcoRI/SphI fragment containing a T7 promoter and bp 22-44 of the IVS.

After linearizing with ScaI, the T7 runoff transcript can be used to produce the ribozyme.

Classification

KINGDOM: Protozoa

References

Cech TR, et al. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods. US Patent 4,987,071 dated Jan 22 1991

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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