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phPMP22-1
phPMP22-1
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B174944
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
phPMP22-1
商品貨號(hào)
B174944
Designations
phPMP22-1
GenBank Number

M94048

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 3.674000024795532
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR1000
Intact vector size: 3.000
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites:
Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
promoter: lac, T7
replicon: ColE1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PMP22
Genomic copy number: unique
Gene name: peripheral myelin protein 22
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 p12-p11.2
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: EcoRI
Insert size (kb): 0.674
Cross references: DNA Seq. Acc.: M94048
Insert lengths(kb): 0.6740000247955322
Gene product: peripheral myelin protein 22 [PMP22]
Insert Size (kb)
0.674
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.75; BglI--3.2, 0.55; EcoRI--3.7; PstI--2.1, 1.6; SacI--3.7.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.09; BglI--0.32.
The gene appears to be located entirely within the Charcot-Marie-Tooth disease (CMT1A) duplication.
Detects genomic restriction fragments of the following sizes (kb): human, HindIII--7.4, 2.5; mouse, HindIII--5.1, 2.1; hamster, HindIII--7.5, 2.8; human, EcoRI--11, 7.9.
Corresponds to nt 147-820 of the published sequence, and contains the complete coding region (nt 50-529 of the published sequence).
Detects transcripts of 1.8, 1.3, and 0.8 kb.
References

Patel PI, et al. The gene for the peripheral myelin protein PMP-22 is a candidate for Charcot-Marie-Tooth disease type 1A. Nat. Genet. 1: 159-165, 1992. PubMed: 1303228

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