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TPO3
TPO3
規格:
價格:
編號:B175041
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TPO3
商品貨號
B175041
Designations
TPO3
GenBank Number

Y00406

培養基
Species
Homo sapiens, human
Depositors
G Vassart
Applications
Codominant segregation observed in 9 (PstI RFLP) and 2 (BglII RFLP) families (38 and 10 individuals, respectively).
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: thyroid
Gene product: thyroid peroxidase [TPO]
Alleles: A2, C1, A1, B1, B2, C2, A1, A2, A3, A4, A5, A6
Insert Size (kb)
3.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Insert contains the following sites separated by (bp): (EcoRI)- 140- ClaI- 263- BglI- 469- BcnI- 142- MboII- 301- MspI- 72- ClaI- 20- AvaII- 950- RsaI- 198- AccI- 455- (EcoRI).
Restriction digests of the clone give the following sizes (kb): XhoI--6.0; BglI--3.5, 1.5, 1.0; HindIII--6.0; EcoRI--3.0, 3.0; AccI--5.4, 0.5.
Codominant segregation observed in 9 (PstI RFLP) and 2 (BglII RFLP) families (38 and 10 individuals, respectively).
Insert contains most of the sequence (from position 77 to the poly(A) signal) of human thyroid peroxidase.
DNA sequence shows 68% homology with the partial porcine thyroid peroxidase sequence.
Also detects a VNTR polymorphism best visualized in BglII and EcoRI digests.
Requires a last stringent wash (0.1X SSC, 65C).
References

Libert F, et al. Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA. Nucleic Acids Res. 15: 6735, 1987. PubMed: 3453124

Massaro G, et al. RFLPs detected at 2 pter-p12 with a thyroid peroxidase cDNA probe, TPO3 (McKusick No. 27450). Nucleic Acids Res. 17: 2155, 1989. PubMed: 2564671

Libert F, et al. Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J. 6: 4193-4196, 1987. PubMed: 3443105

Gilbert Vassart, personal communication

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