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當(dāng)前位置: 首頁 > ATCC代理 > pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1] B175208
pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1]
pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1]
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B175208
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1]
商品貨號(hào)
B175208
Designations
pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1]
GenBank Number

M13974

培養(yǎng)基
Species
Bos taurus, cow
Depositors
A Ullrich
Applications
Detects a human genomic HindIII fragment of 7.5 kb and was used to map the human gene.
Vector
Construct size (kb): 4.619999885559082
Insert
DNA: cDNA
Insert lengths(kb): 1.921000003814697
Tissue: brain
Gene product: protein kinase C, beta 1(protein kinase C, beta I) [PRKCB]
Insert Size (kb)
1.921
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Contains the following restriction sites (with nucleotide positions according to the published sequence): PstI--231, BamHI--623, PstI--865, PstI--1408.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; PstI--3.3, 0.7, 0.6; BamHI--4.4, 0.6; HindIII--5.0.
This is the 5' EcoRI subclone of pbPKC-beta15 and extends from nt 105 to nt 2027 of the published sequence, including most of the coding sequence which extends from nt 65 to nt 2084.
Detects bovine cerebellar mRNA of 8.7, 3.4, and 2.6 kb, and rat brain mRNA of 8.7 and 2.6 kb.
Detects a human genomic HindIII fragment of 7.5 kb and was used to map the human gene.
References

Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548

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