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pbs/PSP94
pbs/PSP94
規格:
價格:
編號:B175686
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pbs/PSP94
商品貨號
B175686
Designations
pbs/PSP94
Species
Homo sapiens, human
培養基
Depositors
M Mbikay
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.5400000214576721
Tissue: prostate
Gene product: microseminoprotein, beta-(prostatic secretory protein) [MSMB]
Insert Size (kb)
0.540
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI/SstI--3.25, 0.54; PstI--3.8; SstI--3.7; XhoI--uncut; KpnI/BamHI--3.2, 0.5; RsaI--2.25, 0.72 (doublet); XhoI--1.5, 1.0, 0.8.
The insert contains EcoRII (4), MboI (2), NsiI (2), HinfI (2), XhoII (1), RsaI (1), TaqI (1), AvaII (1), and HaeIII (2) sites.
The insert consists of 483 bp of cDNA, 53 bp of dG/dC, and 17 bp of poly(A).
The insert encodes the complete prepolypeptide of 114 amino acids and includes 11 bp 5' noncoding and about 128 bp 3' noncoding sequence. There is no AATAAA polyadenylation signal although there is a AGTAAA.
Detects human genomic restriction fragments of the following sizes (kb): EcoRI--3.4 (and a weaker band of 3.0 kb that may be artifactual); BamHI--3.1, 0.5; PvuII--2.8, 0.8, 0.75.
References

Mbikay M, et al. Molecular cloning and sequence of the cDNA for a 94-amino-acid seminal plasma protein secreted by the human prostate. DNA 6: 23-29, 1987. PubMed: 3829888

Nolet S, et al. Rapid evolution of prostatic protein PSP94 suggested by sequence divergence between Rhesus monkey and human cDNAs. Genomics 9: 775-777, 1991. PubMed: 2037304

Majambu Mbikay, personal communication

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