Features: marker(s): ampR promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARS4 replicon: f1 replicon: pMB1 MCS: SacI/.../KpnI centromere: CEN6 coding sequence: lacZ'
Applications
YC-type (centromeric) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.8; EcoRI--3.8; XbaI--3.8.
This vector lacks a selectable marker in yeast. It will contribute many useful features as a base vector when used to construct new vectors that possess yeast selectable markers.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
