Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.7; EcoRI--6.7; BamHI--6.7.
High copy number shuttle expression vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504