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FLHSL
FLHSL
規格:
價格:詢價
編號:B176118
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
FLHSL
商品貨號
B176118
Designations
FLHSL
GenBank Number

X51415

培養基
Species
Rattus norvegicus, rat
Depositors
MC Schotz
Vector
Construct size (kb): 6.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: LIPE
Genomic copy number: unique
Gene name: lipase, hormone-sensitive
Contains complete coding sequence?: Y
Tissue: adipose
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.225
Cross references: DNA Seq. Acc.: X51415
Nucleotides 1-3225 of the insert correspond to
nucleotides 1-3225 of X51415.
Insert lengths(kb): 3.224999904632568
Tissue: adipose
Gene product: lipase, hormone-sensitive [LIPE]
Alleles: S, B,
Insert Size (kb)
3.225
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV/SmaI--3.2, 3.0; EcoRI--3.0, 2.5, 0.8; BamHI--4.4, 2.1.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.16, 1.98; BamHI--2.02; EcoRI--2.49.
References

Holm C, et al. Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3. Science 241: 1503-1506, 1988. PubMed: 3420405

Holm C, et al. Nucleotide sequence of rat adipose hormone sensitive lipase cDNA. Nucleic Acids Res. 16: 9879, 1988. PubMed: 3186461

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