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plambda101
plambda101
規格:
價格:
編號:B176516
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
plambda101
商品貨號
B176516
Designations
plambda101
Species
Homo sapiens, human
培養基
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: hypoxanthine phosphoribosyltransferase pseudogene 1 [HPRTP1]
Alleles: A1, A2
Insert Size (kb)
2.200
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): SalI--4.9; BamHI--4.9; EcoRI--4.9; PstI--3.8, 1.1; EcoRI/BamHI--2.7, 2.2.
The 850 bp HaeIII fragment is free of repeated sequences.
PvuII digests revealed a 5.7 kb fragment with a polymorphic 5.2 kb fragment in 1/33 chromosomes studied.
These RFLPs are also seen with human HPRT cDNA but the picture is more complex because of numerous bands.
The variant HindIII allele segregates independently of the variant PvuII allele.
The 850 bp HaeIII fragment detects the following genomic fragments (kb): EcoRI--8.8; HindIII--9.8; BamHI--3.4 kb.
This clone was derived from Hulambda10.
Enzyme(s) not detecting polymorphism: TaqI, MspI, PstI, BstNI, XbaI, SacI, HincII, StuII, MboI, EcoRV.
References

Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358

Dobrovic A, et al. A HindIII RFLP for the HPRT pseudogene on chromosome 3 (HPRTP1). Nucleic Acids Res. 15: 1346, 1987. PubMed: 2881273

Pragna I Patel, personal communication

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