| Vector |
Construct size (kb): 4.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP64
Intact vector size: 2.999
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->
MCS: HindIII...EcoRI, ->
enhancer: none
replicon: pMB1
marker(s): ampR
terminator: none
Cross references: |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; HindIII--4.2; EcoRI--4.2; Pvul--4.2; PstI--4.2; EcoRI/PstI--3.2, 0.9. This probe can be used to detect Actinobacillus actinomycetemcomitans (proposed to be reclassified as Haemophilus actinomycetemcomitans), a causative agent of juvenile periodontitis. This probe does not hybridize to Haemophilus aphrophilus (strains 621, 626, 654, 655, H77, H80, H81, ATCC 13252, ATCC 19415, NCTC 5906), H. paraprophilus (strains H76, H78, ATCC 29241), or H. influenza (strains H9, H104). This probe hybridizes to A. actinomycetemcomitans, strains Y4, 511, 652, ATCC 29522, ATCC 29523, ATCC 29524, 650, 651, 652, 2043, 2097, 2112, N27, NCTC 9710. |
