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pSP64-Aa198
pSP64-Aa198
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B176583
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pSP64-Aa198
商品貨號(hào)
B176583
Designations
pSP64-Aa198
Species
Haemophilus actinomycetemcomitans (Klinger) Potts et al.
培養(yǎng)基
Depositors
OmniGene, Inc., MC Chen, OmniGene, Inc.
Applications
This probe can be used to detect Actinobacillus actinomycetemcomitans (proposed to be reclassified as Haemophilus actinomycetemcomitans), a causative agent of juvenile periodontitis.
Vector
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP64
Intact vector size: 2.999
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->
MCS: HindIII...EcoRI, ->
enhancer: none
replicon: pMB1
marker(s): ampR
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Haemophilus actinomycetemcomitans
Gene name: genus specific DNA
Contains complete coding sequence?: U
Strain: Y4
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 1.2
Cross references:
Insert lengths(kb): 1.200000047683716
Gene product: genus specific DNA
Insert Size (kb)
1.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; HindIII--4.2; EcoRI--4.2; Pvul--4.2; PstI--4.2; EcoRI/PstI--3.2, 0.9.
This probe can be used to detect Actinobacillus actinomycetemcomitans (proposed to be reclassified as Haemophilus actinomycetemcomitans), a causative agent of juvenile periodontitis.
This probe does not hybridize to Haemophilus aphrophilus (strains 621, 626, 654, 655, H77, H80, H81, ATCC 13252, ATCC 19415, NCTC 5906), H. paraprophilus (strains H76, H78, ATCC 29241), or H. influenza (strains H9, H104).
This probe hybridizes to A. actinomycetemcomitans, strains Y4, 511, 652, ATCC 29522, ATCC 29523, ATCC 29524, 650, 651, 652, 2043, 2097, 2112, N27, NCTC 9710.
References

Chen MC, et al. Detection of human oral cells by nucleic acid hybridization. US Patent 4,866,167 dated Sep 12 1989

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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