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Construct size (kb): 35.40000152587891

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: FUR1
Gene name: uracil phosphoribosyltransferase
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: ACT5
Gene name: centractin
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: YCK1
Gene name: casein kinase 1 homolog
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: SPS100
Gene name: sporulation-specific wall maturation protein
Contains complete coding sequence?: Y
Chromosome: VIII
Genome: Saccharomyces cerevisiae
Gene symbol: RPL4B
Other gene symbols: MAK18
Gene name: ribosomal protein L41
Contains complete coding sequence?: Y
Chromosome: VIII
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 28.995
Cross references: DNA Seq. Acc.: U10397
DNA Seq. Acc.: U10398

Insert lengths(kb): 28.9950008392334

Gene product: uracil phosphoribosyltransferase [ACT5]

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 4.8, 4.3, 3.4, 2.9 (doublet), 2.6, 2.5 and smaller bands.

Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.579 (vector), 4.723, 4.194, 3.339, 2.994, 2.871, 2.652, 2.478, 1.668, 1.465, 1.338, 1.334, 1.162 (vector), 0.998, 0.871, 0.788, 0.713, 0.475, 0.445, 0.407.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Corresponds to nt 357767-386762 of the sequence of chromosome VIII.