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pRY255
pRY255
規格:
價格:詢價
編號:B176656
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRY255
商品貨號
B176656
Designations
pRY255
Depositors
RR Yocum
培養基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli YMC9
Vector Information
Size (kb): 17.8999996185302700
Vector: pRY255 (plasmid)
Promoters: Promoter CYC1
Construction: pRY253
Marker(s):ampR G418R
Construct size (kb): 17.89999961853027
Features: marker(s): G418R
marker(s): ampR
other: HO
promoter: CYC1
replicon: pMB1
coding sequence: GAL1/lacZ fusion
Applications
YI-type (integrating) shuttle vector
vector permitting positive selection for integration
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0, 7.4; KpnI--19.0; SalI--19.0.
A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter. Expresses a GAL1/lacZ fusion product allowing color selection.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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