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Ca Ski
Ca Ski
規格:
價格:詢價
編號:B176686
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Ca Ski
商品貨號
B176686
Organism
Homo sapiens, human
Tissue
cervix; Derived fom metastatic site: small intestine
培養基
Cell Type
epithelial cell (CRM)
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2
[Cells contain human papilloma virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
epidermoid carcinoma
Age
40 years adult
Gender
female
Ethnicity
Caucasian
Applications
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. 
Intended Use

Certified Reference Material produced under an ISO 17034 accredited process.


    ACLASS Accredited Reference Material Producer AR-1384

Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established from cells from a metastasis in the small bowel mesentery.
Clinical Data
female
Caucasian
40 years adult
Genes Expressed
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Cellular Products
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Comments

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18.

NOTE: Virology taxonomy has discontinued use of the family name "Papovaviridae," which includes the term "Papovavirus." Instead, this former family name, "Papovaviridae," has now been split into two distinct and separate families, "Papillomaviridae" and "Polyomaviridae." As such, all cultures containing HPV should be noted to contain "Papillomavirus" (signifying it's origin from the "Papillomaviridae" family) instead of the former "Papovavirus."

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
Isoenzymes
G6PD, B
Name of Depositor
RA Pattillo
Deposited As
Homo sapiens
References

Pattillo RA, et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. PubMed: 867042

Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

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