| 產(chǎn)品名稱 |
pLW46 |
| 商品貨號 |
B176714 |
| Designations |
pLW46 |
| GenBank Number |
I02223
|
| Species |
Homo sapiens, human |
| Depositors |
Cetus Corp., A Belt, Cetus Corp. |
| Vector |
Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: BanII
Vector end: HindIII
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IL2
Genomic copy number: unique
Gene name: interleukin 2
Contains complete coding sequence?: U
Chromosome: 4; Localization: 4 q26-q27
Tissue: lymphocyte
Type of DNA: cDNA
Insert end: HindIII
Insert end: BanII
Insert size (kb):
Cross references: DNA Seq. Acc.: I02223 Tissue: lymphocyte Gene product: interleukin 2 [IL2] |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains XbaI, PstI, and DdeI sites. The C to G substitution at nucleotide 374 changes the amino acid sequence from Cys125 to Ser125 and introduces the DdeI site. The amino terminal sequence is Met-Pro-Thr-Ser, with Met removed during processing. This lacks the native N-terminal Ala. The Escherichia coli trp promoter is contained in an EcoRI/HindIII fragment. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References |
Wang A, et al. Site-specific mutagenesis of the human interleukin-2 gene: Structure-function analysis of the cysteine residues. Science 224: 1431-1433, 1984. PubMed: 6427925
Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985
Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987
Barbara Weiffenbach, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
