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pLW46
pLW46
規格:
價格:詢價
編號:B176714
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLW46
商品貨號
B176714
Designations
pLW46
GenBank Number

I02223

培養基
Species
Homo sapiens, human
Depositors
Cetus Corp., A Belt, Cetus Corp.
Vector
Construct size (kb): 0.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTRP3
Intact vector size: 4.460
Type of vector: plasmid
Vector end: BanII
Vector end: HindIII
Cloning sites: HindIII
Polylinker sites:
Other unique sites: BamHI
Construction: pVH153, pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: trp, ->
ribosome-binding site: , ->
restriction site: HindIII, ->
marker(s): tetR, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IL2
Genomic copy number: unique
Gene name: interleukin 2
Contains complete coding sequence?: U
Chromosome: 4; Localization: 4 q26-q27
Tissue: lymphocyte
Type of DNA: cDNA
Insert end: HindIII
Insert end: BanII
Insert size (kb):
Cross references: DNA Seq. Acc.: I02223
Tissue: lymphocyte
Gene product: interleukin 2 [IL2]
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains XbaI, PstI, and DdeI sites.
The C to G substitution at nucleotide 374 changes the amino acid sequence from Cys125 to Ser125 and introduces the DdeI site. The amino terminal sequence is Met-Pro-Thr-Ser, with Met removed during processing. This lacks the native N-terminal Ala.
The Escherichia coli trp promoter is contained in an EcoRI/HindIII fragment.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References

Wang A, et al. Site-specific mutagenesis of the human interleukin-2 gene: Structure-function analysis of the cysteine residues. Science 224: 1431-1433, 1984. PubMed: 6427925

Lin LS, et al. Human recombinant interleukin-2-muteins. US Patent 4,518,584 dated May 21 1985

Gelfand DH, et al. Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element. US Patent 4,666,848 dated May 19 1987

Barbara Weiffenbach, personal communication

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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